MOS CURRENT MODE LOGIC (MCML) ANALYSIS FOR QUIET DIGITAL CIRCUITRY AND CREATION OF A STANDARD CELL LIBRARY FOR REDUCING THE DEVELOPMENT TIME OF MIXED-SIGNAL CHIPS

Many modern digital systems use forms of CMOS logical implementation due to the straight forward design nature of CMOS logic and minimal device area since CMOS uses fewer transistors than other logic families. To achieve high-performance requirements in mixed-signal chip development and quiet, noiseless circuitry, this thesis provides an alternative toCMOSin the form of MOS Current Mode Logic (MCML). MCML dissipates constant current and does not produce noise during value changing in a circuit CMOS circuits do. CMOS logical networks switch during clock ticks and with every device switching, noise is created on the supply and ground to deal with the transitions. Creating a noiseless standard cell library with MCML allows use of circuitry that uses low voltage switching with 1.5V between logic levels in a quiet or mixed-signal environment as opposed to the full rail to rail swinging of CMOS logic. This allows cohesive implementation with analog circuitry on the same chip due to constant current and lower switching ranges not creating rail noise during digital switching. Standard cells allow for the Cadence tools to automatically generate circuits and Cadence serves as the development platform for the MCML standard cells. The theory surrounding MCML is examined along with current and future applications well-suited for MCML are researched and explored with the goal of highlighting valid candidate circuits for MCML. Inverters and NAND gates with varying current drives are developed to meet these specialized goals and are simulated to prove viability for quiet, mixed-signal applications. Analysis and results show that MCML is a superior implementation choice compared toCMOSfor high speed and mixed signal applications due to frequency independent power dissipation and lack of generated noise during operation. Noise results show rail current deviations of 50nA to 300nA during switching over an average operating current of 20µA to 80µA respectively. The multiple order of magnitude difference between noise and signal allow the MCML cells to dissipate constant power and thus perform with no noise added to a system. Additional simulated results of a 31-stage ring oscillator result in a frequency for MCML of 1.57GHz simulated versus the 150.35MHz that MOSIS tested on a fabricated 31-stage CMOS oscillator. The layouts designed for the standard cell library conform to existing On Semiconductor ami06 technology dimensions and allow for design of any logical function to be fabricated. The I/O signals of each cell operate at the same input and output voltage swings which allow seamless integration with each other for implementation in any logical configuration

SoluProt: prediction of soluble protein expression in Escherichia coli

Motivation: Poor protein solubility hinders the production of many therapeutic and industrially useful proteins. Experimental efforts to increase solubility are plagued by low success rates and often reduce biological activity. Computational prediction of protein expressibility and solubility in Escherichia coli using only sequence information could reduce the cost of experimental studies by enabling prioritization of highly soluble proteins. Results: A new tool for sequence-based prediction of soluble protein expression in E.coli, SoluProt, was created using the gradient boosting machine technique with the TargetTrack database as a training set. When evaluated against a balanced independent test set derived from the NESG database, SoluProt's accuracy of 58.5% and AUC of 0.62 exceeded those of a suite of alternative solubility prediction tools. There is also evidence that it could significantly increase the success rate of experimental protein studies

Seismic detection of the martian core by InSight

A plethora of geophysical, geo- chemical, and geodynamical observations indicate that the terrestrial planets have differentiated into silicate crusts and mantles that surround a dense core. The latter consists primarily of Fe and some lighter alloying elements (e.g., S, Si, C, O, and H) [1]¿. The Martian meteorites show evidence of chalcophile element depletion, suggesting that the otherwise Fe-Ni- rich core likely contains a sulfide component, which influences physical state

Energy Harvesting Of Human Kinetic Movement

Development of kinetic energy scavenging applications from the human body necessitates additional research to assist in designating a mounting position for a potential device. A data acquisition system adequately provides a parametric average power comparison among four locations on the body (waist, upper arm, hand, and calf) for both a male and female subject. Experimentally, the hand-held device provided the highest average power. Thus, subsequent investigation at set speeds provides further analysis of the output’s characteristically linear behavior. The physical energy-harvesting device features a plastic tube casing wrapped with the stationary coiled wire through which a neodymium magnet oscillates. While the data delivers a practical comparison for a mounting point and angle, size and power output may increase or decrease depending on variable device parameters

Seismic detection of the Martian core by InSight

vEGU21: Gather Online | 19–30 April 2021Introduction: A plethora of geophysical, geo-chemical, and geodynamical observations indicate that the terrestrial planets have differentiated into silicate crusts and mantles that surround a dense core. The latter consists primarily of Fe and some lighter alloying elements (e.g., S, Si, C, O, and H). There is strong evidence from measurements of the tidal deformation of the planet that the core of Mars is presently liquid. The InSight mission aims at constraining these numbers via the RISE radio tracking experiment, and the SEIS seismic package. We used data recorded by SEIS for high SNR marsquakes between March 2019 and July 2020. The InSight Marsquake Service located these events in the distance range 27-40 degrees, based on identification of P- and S-body waves. Later studies identified a number of secondary, surface-reflected phases, which were used to constrain the upper mantle. We build upon the velocity models derived from these phase picks to constrain the time window in which to look for shear waves reflected from the core mantle boundary. Since shear waves cannot propagate in a fluid medium, the core mantle boundary (CMB) acts as a polarization filter, which fully reflects horizontally polarized shear waves back into the mantle. Shear waves reflected from the CMB, called ScS, are therefore expected to have a predominantly horizontal polarization at the receiver, with an azimuth orthogonal to the source direction. In this distance range, ScS is separated in time from any other body wave phase and therefore well-observable. Methods: We follow a two-step approach: 1. Confirm seismic arrivals as ScS, based on existing mantle velocity models. 2. Pick precise arrival times and invert those for mantle profiles and core size, constrained by mineralogy, moment of inertia and average density of the planet. Results: The inversion of travel times constrains the core radius to the upper end of pre-mission geophysics-based estimates. This value is compatible with estimates from the geodetic experiment RISE onboard and implies that a lower mantle is unlikely to be present. Moreover, a large core has important implications for core composition. Average retrieved core density is 6 g/cm^3, which implies that for a (Fe-Ni)-S composition, a sulfur content in excess of 18% is required. This is above the eutectic composition observed experimentally with potentially profound implications for the future crystallization of the Martian core, subject to further laboratory research of Fe-S data under core conditions. All ScS candidate phases that were observed show significant seismic energy and a relatively flat spectrum above 0.1 Hz, which implies a low seismic attenuation throughout the mantle. The spectral character of direct S-phases for the distant-most events is consistent with that of the ScS-phases for more nearby events, which supports the identification of the arrivals as core-reflected

Evaluation of a novel screening method for fetal aneuploidy using cell-free DNA in maternal plasma

Objective: To evaluate the test performance of a novel sequencing technology using molecular inversion probes applied to cell-free DNA screening for fetal aneuploidy. Methods: Two cohorts were included in the evaluation; a risk-based cohort of women receiving diagnostic testing in the first and second trimesters was combined with stored samples from pregnancies with fetuses known to be aneuploid or euploid. All samples were blinded to testing personnel before being analyzed, and validation occurred after the study closed and results were merged. Results: Using the new sequencing technology, 1414 samples were analyzed. The findings showed sensitivities and specificities for the common trisomies and the sex chromosome aneuploidies at >99% (Trisomy 21 sensitivity 99.2 CI 95.6-99.2; specificity 99.9 CI 99.6-99.9). Positive predictive values among the trisomies varied from 85.2% (Trisomy 18) to 99.0% (Trisomy 21), reflecting their prevalence rates in the study. Comparisons with a meta-analysis of recent cell-free DNA screening publications demonstrated equivalent test performance. Conclusion: This new technology demonstrates equivalent test performance compared with alternative sequencing approaches, and demonstrates that each chromosome can be successfully interrogated using a single probe

Seismic detection of the Martian core by InSight

Introduction: A plethora of geophysical, geo-chemical, and geodynamical observations indicate that the terrestrial planets have differentiated into silicate crusts and mantles that surround a dense core. The latter consists primarily of Fe and some lighter alloying elements (e.g., S, Si, C, O, and H). There is strong evidence from measurements of the tidal deformation of the planet that the core of Mars is presently liquid. The InSight mission aims at constraining these numbers via the RISE radio tracking experiment, and the SEIS seismic package. We used data recorded by SEIS for high SNR marsquakes between March 2019 and July 2020. The InSight Marsquake Service located these events in the distance range 27-40 degrees, based on identification of P- and S-body waves. Later studies identified a number of secondary, surface-reflected phases, which were used to constrain the upper mantle. We build upon the velocity models derived from these phase picks to constrain the time window in which to look for shear waves reflected from the core mantle boundary. Since shear waves cannot propagate in a fluid medium, the core mantle boundary (CMB) acts as a polarization filter, which fully reflects horizontally polarized shear waves back into the mantle. Shear waves reflected from the CMB, called ScS, are therefore expected to have a predominantly horizontal polarization at the receiver, with an azimuth orthogonal to the source direction. In this distance range, ScS is separated in time from any other body wave phase and therefore well-observable. Methods: We follow a two-step approach: 1. Confirm seismic arrivals as ScS, based on existing mantle velocity models. 2. Pick precise arrival times and invert those for mantle profiles and core size, constrained by mineralogy, moment of inertia and average density of the planet. Results: The inversion of travel times constrains the core radius to the upper end of pre-mission geophysics-based estimates. This value is compatible with estimates from the geodetic experiment RISE onboard and implies that a lower mantle is unlikely to be present. Moreover, a large core has important implications for core composition. Average retrieved core density is 6 g/cm^3, which implies that for a (Fe-Ni)-S composition, a sulfur content in excess of 18% is required. This is above the eutectic composition observed experimentally with potentially profound implications for the future crystallization of the Martian core, subject to further laboratory research of Fe-S data under core conditions. All ScS candidate phases that were observed show significant seismic energy and a relatively flat spectrum above 0.1 Hz, which implies a low seismic attenuation throughout the mantle. The spectral character of direct S-phases for the distant-most events is consistent with that of the ScS-phases for more nearby events, which supports the identification of the arrivals as core-reflected